Microbiology Essay Example | Topics and Well Written Essays - 1500 words - 1

Microbiology - Essay Example An evaluation of the total viable count and the presence of pathogens in the uncooked chicken were taken into account. The kitchen sponge and the hand wash water of the chicken were considered for the analysis. The analysis for the presence of the pathogenic micro organisms will help us to overcome the toxicity levels. (Mattick et al. 2003; Mattick et al. 2003a). A lot of tests were done to determine the presence of the bacterial load in the micro organisms. Some of them include Gram staining, Catalase test, Oxidase test, coagulase test, API Strip test, the effect of disinfectant hypochloride, cleaning agents and morphological tests. The presence of the pathogens is found using the biochemical tests and the API strip test. The differential staining of the bacteria helps to identify the unknown bacteria present in the sample. Before all the tests the samples are serially diluted and the 4th (10-4) and 5th diluted (10-5) sample are used for the analysis. The API 20E strip consists of 20 individual and miniature tests that contain the reagents for the identification of the metabolic capabilities and specifically the Enterobacteraceae family. (Butler et al. 1975). For the antimicrobial susceptibility testing, the Iso-Sensitest Agar (ISA) media was used. To test the presence o f enterobacteriaceae, the ISA media is enough according to the BSAC. The Molten agar was poured into the sterile Petri plates and they are dried to remove the excess moisture. To these plates, the antimicrobials of choice are used to such that it diffuses into the medium and they interact very well with the micro organism incorporated. The disc diffusion method of AST is the most commonest and effective method for the detection of the microorganisms in the sample. The inoculums was prepared and inoculated in the medium. The plates were incubated at 35-37 degree Celsius for at least 24 hours.